CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein

被引：0
作者：
Lichun Tang
Yanting Zeng
Hongzi Du
Mengmeng Gong
Jin Peng
Buxi Zhang
Ming Lei
Fang Zhao
Weihua Wang
Xiaowei Li
Jianqiao Liu
机构：
[1] Beijing Institute of Radiation Medicine,State Key Laboratory of Proteomics, Beijing Proteome Research Center
[2] National Center for Protein Sciences Beijing,Center for Reproductive Medicine, The Third Affiliated Hospital
[3] Guangzhou Medical University,National Center for International Research of Biological Targeting Diagnosis and Therapy, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy
[4] Guangxi Medical University,Department of Cardiology
[5] Houston Fertility Institute,undefined
[6] Bayi Hospital Affiliated Nanjing University of Chineses Medicine,undefined
来源：
Molecular Genetics and Genomics | 2017年 / 292卷
关键词：
CRISPR/Cas9; Homology-directed repair (HDR); Cas9 protein; Gene modification; Human zygotes;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research.
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页码：525 / 533
页数：8
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