The effect of osmotic potential on anther culture in spring wheat (Triticum aestivum)

被引：0
作者：
Tae-Jin Kang
Moon-Sik Yang
Edward L. Deckard
机构：
[1] Chonbuk National University,Institute of Basic Sciences
[2] Chonbuk National University,Division of Biological Sciences and the Research Center for Bioactive Materials
[3] North Dakota State University,Department of Plant Sciences
来源：
Plant Cell, Tissue and Organ Culture | 2003年 / 75卷
关键词：
cv. Chris; cv. Pavon; fertility; ploidy; polyethylene glycol; sucrose;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study was conducted to determine the effect of osmotic potential in a modified 85D12 medium on both callus induction and plant regeneration in the anther culture of two wheat genotypes, cv. Chris and cv. Pavon. Altering the medium osmotic potential by changing the carbohydrate source and concentration or by adding a non-metabolized osmoticum appeared to have the greatest potential for improving anther-derived green plant production. The medium osmotic potentials were varied (-0.67 to −2.30 MPa) by altering sucrose and PEG concentration. Both osmotica affected callus production, with −0.9 to −1.4 MPa media producing the most calluses. Callus production depended on genotype and osmoticum. Only PEG concentration affected green plant regeneration. The greatest number of green plants (11.5 plants per 100 anthers in cv. Chris) was obtained with 0.0125 M of PEG. This experiment suggested that a low level of PEG in the medium was beneficial for producing green plants from wheat anthers.
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页码：35 / 40
页数：5
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