Evaluation of salt tolerance of transgenic tobacco plants bearing the P5CS1 gene of Arabidopsis thaliana

被引：0
作者：
S. M. Ibragimova
E. A. Trifonova
E. A. Filipenko
V. K. Shymny
机构：
[1] Russian Academy of Sciences,Institute of Cytology and Genetics Siberian Branch
[2] Novosibirsk State University,Department of Cytology and Genetics
来源：
Russian Journal of Genetics | 2015年 / 51卷
关键词：
genetic engineering; proline metabolism genes; resistance; stress;
D O I：
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中图分类号：
学科分类号：
摘要：
Arabidopsis thaliana Δ1-pyrroline-5-carhoxylate synthetase 1 (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. Petit Havana SR-1 (Nicotiana tabacum L.) plants. It is shown that the constitutive level of proline in the transgenic plants T0 exceeds that of the SR1 reference line by 1.5 to 4 times. Under conditions of salt stress (200, 300 mM NaCl) T1-generation transgenic plants in early stages of development formed a large biomass, developed more quickly, and had a higher rate of root growth compared to the control, which confirms the involvement of the P5CS1 gene in molecular mechanisms of stress resistance in plants.
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页码：1181 / 1188
页数：7
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