Morphological analysis of the sheathed flagellum of Brucella melitensis

被引：25
作者：
Ferooz J. [1 ,2 ]
Letesson J.-J. [1 ]
机构：
[1] Unité de Recherche en Biologie Moléculaire (URBM), Facultés Universitaires Notre-Dame de la Paix (FUNDP), B-5000 Namur
[2] GlaxoSmithKline Biologicals, B-1300 Wavre
来源：
BMC Research Notes | / 3卷 / 1期
基金：
澳大利亚研究理事会;
关键词：
Brucella Melitensis; Flagellar Gene; Bended Structure; Early Exponential Growth Phase; Flagellar Assembly;
D O I：
10.1186/1756-0500-3-333
中图分类号：
学科分类号：
摘要：
Background. It was recently shown that B. melitensis is flagellated. However, the flagellar structure remains poorly described. Findings. We analyzed the structure of the polar sheathed flagellum of B. melitensis by TEM analysis and demonstrated that the Ryu staining is a good method to quickly visualize the flagellum by optical microscopy. The TEM analysis demonstrated that an extension of the outer membrane surrounds a filament ending by a club-like structure. The ftcR, fliF, flgE and fliC flagellar mutants still produce an empty sheath. Conclusions. Our results demonstrate that the flagellum of B. melitensis has the characteristics of the sheathed flagella. Our results also suggest that the flagellar sheath production is not directly linked to the flagellar structure assembly and is not regulated by the FtcR master regulator. © 2010 Letesson et al; licensee BioMed Central Ltd.
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