Spectrofluorimetric quantification of antibiotic drug concentration in bacterial cells for the characterization of translocation across bacterial membranes

被引：0

作者：

Julia Vergalli

Estelle Dumont

Jelena Pajović

Bertrand Cinquin

Laure Maigre

Muriel Masi

Matthieu Réfrégiers

Jean-Marie Pagés

机构：

[1] UMR_MD1,

[2] U-1261,undefined

[3] Aix-Marseille Univ.,undefined

[4] INSERM,undefined

[5] SSA,undefined

[6] IRBA,undefined

[7] MCT,undefined

[8] DISCO Beamline,undefined

[9] Synchrotron Soleil,undefined

[10] ,undefined

[11] L'Orme des Merisiers,undefined

[12] Faculty of Physics,undefined

[13] University of Belgrade,undefined

[14] Present address: Bertrand Cinquin,undefined

[15] LBPA,undefined

[16] ENS CACHAN,undefined

[17] Cachan,undefined

[18] France.,undefined

来源：

Nature Protocols | 2018年 / 13卷

关键词：

D O I：

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中图分类号：

学科分类号：

摘要：

This protocol provides three options for quantifying antibiotic drug concentration in bacterial cells: spectrofluorimetry, microspectrofluorimetry, and kinetics microspectrofluorimetry methods. These approaches are used, respectively, to determine the antibiotic concentration in bacterial populations, in individual cells, and in individual cells across a time course of measurements.

引用

页码：1348 / 1361

页数：13

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