Differential effects of tumor–platelet interaction in vitro and in vivo in glioblastoma

被引:0
作者
Marc A. Brockmann
Birte Bender
Elena Plaxina
Ingo Nolte
Ralf Erber
Katrin Lamszus
Christoph Groden
Lothar Schilling
机构
[1] University of Heidelberg,Department of Neuroradiology, Medical Faculty Mannheim
[2] Leopold-Franzens University of Innsbruck,Department of Otorhinolaryngology
[3] University of Heidelberg,Department of Orthodontics and Dentofacial Orthopaedics, Dental School
[4] University Hospital Hamburg-Eppendorf,Department of Neurosurgery
[5] University of Heidelberg,Division of Neurosurgical Research, Medical Faculty Mannheim
来源
Journal of Neuro-Oncology | 2011年 / 105卷
关键词
Angiogenesis; Platelets; Glioblastoma; Thrombosis; Animal model;
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摘要
An elevated platelet count is considered an independent predictor of short survival in glioblastoma and various other tumor entities. Prothrombotic activity of the tumor microcirculation resulting in platelet activation and release of cytokines from activated platelets has been suggested to play a role. This study was designed to analyze the effects of platelet-released cytokines on glioblastoma and endothelial cell proliferation and migration in vitro, and the influence of platelet count on glioblastoma growth and angiogenesis in vivo. In cultured human glioblastoma, umbilical cord and cerebral microvascular endothelial cells platelet-released cytokines significantly stimulated proliferation and migration as well as sprouting and formation of capillary-like structures. In vivo, glioblastoma cells were implanted in mice followed by platelet depletion starting 1 or 8 days later. Tumor volume, proliferative index, and vessel density analyzed 14 days after engraftment did not differ between animals with a normal and a low platelet count. Likewise, no effect of platelet depletion over 20 days upon the volume of intracerebrally growing tumors was observed in mice. Additionally, proliferative activity and vessel density determined in tumor samples from patients operated upon glioblastoma did not show any correlation with the patients’ preoperative platelet count. Thus, we conclude that distinct proliferation- and chemotaxis-stimulating effects of platelet-derived cytokines can be achieved in vitro, while the platelet count does not exert a major influence on tumor growth and tumor angiogenesis in GBM in vivo.
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页码:45 / 56
页数:11
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