Molecular characterisation of porcine reproductive and respiratory syndrome virus from pigs in Kerala

被引：2

作者：

Chaithra G. ^{[1
]}

Ravishankar C. ^{[1
]}

Sebastian S.R. ^{[1
]}

Rajasekhar R. ^{[1
]}

Anoopraj R. ^{[2
]}

Mani B.K. ^{[1
]}

Sumod K. ^{[1
]}

Deorao C.V. ^{[1
]}

Logeshwaran G. ^{[1
]}

Nandhakumar D. ^{[1
]}

John K. ^{[1
]}

机构：

[1] Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi P.O., Wayanad, 673576, Kerala

[2] Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi P.O., Wayanad, 673576, Kerala

来源：

VirusDisease | 2020年 / 31卷 / 4期

关键词：

Nsp2; ORF5; ORF7; Phylogeny; PRRSV; RT-PCR;

D O I：

10.1007/s13337-020-00634-7

中图分类号：

学科分类号：

摘要：

Porcine reproductive and respiratory syndrome (PRRS) caused by an arterivirus is characterised by reproductive disorders in sows, and post-weaning pneumonia and growth reduction in piglets. Though the virus has been detected in Kerala, no systematic study has been carried out to ascertain its genotype and molecular epidemiology. In the present study, 7 PRRS virus (PRRSV) positive samples collected from incidences of PRRS in Kerala during 2017–2019 were subjected to ORF5, ORF7 and Nsp2 gene based reverse transcription polymerase chain reaction and the specific amplicons generated were sequenced. On BLAST analysis it was revealed that all the sequences were of genotype 2 (North American genotype). Phylogenetic analysis of ORF5 sequences, grouped them under subgenotype 4 with close clustering with other isolates from Kerala, Mizoram and Assam. Nsp2 gene sequence based phylogenetic analysis grouped the isolates under subgenotype 3 with similarities to isolates from Mizoram. Phylogenetic analysis based on ORF7, clustered the isolates under study with PRRSV isolates from Mizoram and Meghalaya. In Nsp2 sequences, a 30 amino acid discontinuous deletion was observed. On analysis of amino acid sequences of ORF5 of Kerala isolates and those from India, it was seen that the Kerala isolates showed closer similarity to PRRSV isolates from Assam than to the other Indian isolates. The study reveals that PRRSV strains prevalent in Kerala share close relationship with other PRRSV isolates in India. This may be due to spread of the virus from these regions to Kerala due to animal movement. Concerted efforts should be undertaken to check unauthorized animal movement to control spread of this economically important disease. © 2020, Indian Virological Society.

引用

页码：560 / 565

页数：5

共 20 条

[1] An T.Q., Zhou Y.J., Liu G.Q., Tian Z.J., Li J., Qiu H., Tong G.Z., Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: coexistence of two NA-subgenotypes with great diversity, Vet Microbiol, 123, pp. 43-52, (2007)
[2] Deng Y., Pan Y., Wang D., Zhou Q., Bi Y., Chen F., Song Y., Complete genome sequence of porcine reproductive and respiratory syndrome virus strain QY2010 reveals a novel subgroup emerging in China, J Virol, 86, pp. 7719-7720, (2012)
[3] Do H.Q., Trinh D.T., Nguyen T.L., Vu T.T., Than D.D., Lo T.V., Yeom M., Song D., Choe S., An D.J., Le V.P., Molecular evolution of type 2 porcine reproductive and respiratory syndrome viruses circulating in Vietnam from 2007 to 2015, BMC Vet Res, 12, (2016)
[4] Dortmans J.C.F.M., Buter G.J., Dijkman R., Houben M., Duinhof T.F., Molecular characterization of type 1 porcine reproductive and respiratory syndrome viruses (PRRSV) isolated in the Netherlands from 2014 to 2016, PLoS ONE, 14, pp. 1-13, (2019)
[5] Guo Z., Chen X.X., Li R., Qiao S., Zhang G., The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective, J Virol, 15, (2018)
[6] Hu H., Li X., Zhang Z., Shuai J., Chen N., Liu G., Fang W., Porcine reproductive and respiratory syndrome viruses predominant in southeastern China from 2004 to 2007 were from a common source and underwent further divergence, Arch Virol, 154, pp. 391-398, (2009)
[7] Virus Taxonomy: 2019 Release
[8] Kang H., Yu J.E., Shin J.E., Kang A., Kim W.I., Lee C., Lee J., Cho I.S., Choe S.E., Cha S.H., Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016, BMC Vet Res, 14, (2018)
[9] Kumar S., Stecher G., Li M., Knyaz C., Tamura K., MEGA X: molecular evolutionary genetics analysis across computing platforms, Mol Biol Evol, 35, pp. 1547-1549, (2018)
[10] Li Y., Wang X., Bo K., Wang X., Tang B., Yang B., Jiang W., Jiang P., Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China, Vet J, 174, pp. 577-584, (2007)

← 1 2 →