Differential Regulation of the Two Metallothionein Genes in Common Carp

被引：0
作者：
Edit Hermesz
Anett P. Gazdag
K. Said Ali
J. Nemcsók
Magdolna Ábrahám
机构：
[1] University of Szeged,Department of Biochemistry, Faculty of Science
来源：
Acta Biologica Hungarica | 2002年 / 53卷
关键词：
Carp; gene expression; metallothionein mRNAs; metal treatment; RT-PCR;
D O I：
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学科分类号：
摘要：
The expression of two metallothionein (MT) genes was followed in carp (Cyprinus carpio) in vivo during exposure to As and Cu. Changes in the levels of MT-1 and MT-2 mRNA in the liver and kidney were detected by semi-quantitative RT-PCR. The inducibility of the two MT isoforms was tissue- and metal-specific. Regardless of whether As or Cu was applied, the liver was more responsive than the kidney. Copper influenced the expression of both isoforms: the MT-1 and MT-2 mRNA levels increased in both the liver and the kidney in a time- and dose-dependent manner. Arsenic affected mostly the MT-2 expression, while the level of the MT-1 transcript did not change significantly in either organ.
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页码：343 / 350
页数：7
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