Study of the reaction of kidney collecting duct principal cells to hypotonic shock. Experiment and mathematical model

被引：2

作者：

Ilyaskin A.V. ^{[1
]}

Baturina G.S. ^{[1
]}

Medvedev D.A. ^{[2
,3
]}

Ershov A.P. ^{[2
,3
]}

Solenov E.I. ^{[1
,3
]}

机构：

[1] Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences

[2] Lavrent'ev Institute of Hydrodynamics, Siberian Branch, Russian Academy of Sciences

[3] Novosibirsk State University

来源：

Biophysics | 2011年 / 56卷 / 3期

基金：

俄罗斯基础研究基金会;

关键词：

cell volume regulation; kidney; mathematical model;

D O I：

10.1134/S0006350911030092

中图分类号：

学科分类号：

摘要：

The reaction of rat kidney collecting duct principal cells to hypotonic shock was studied. The changes in cell relative volume were measured using fluorescent dye calcein, and a mathematical model based on our experimental results was developed. It was shown that regulatory volume decrease is mainly provided by significant release of osmolytes from the cell and decrease of the plasma membrane water permeability. Using our model, we calculated the membrane water permeability and found it to decrease from 2 · 10-1 to 2 · 10-2 cm/s. We conclude that for effective RVD to occur, a dramatic increase in the membrane permeability to K+, Cl- and organic anions is necessary. © 2011 Pleiades Publishing, Ltd.

引用

页码：516 / 524

页数：8

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