Molecular and phylogenetic analysis of HIV-1 variants circulating in Italy

被引：7

作者：

Buonaguro L. ^{[1
]}

Petrizzo A. ^{[1
]}

Tagliamonte M. ^{[1
]}

Vitone F. ^{[2
]}

Re M.C. ^{[2
,3
]}

Pilotti E. ^{[4
]}

Casoli C. ^{[5
]}

Sbreglia C. ^{[6
]}

Perrella O. ^{[6
]}

Tornesello M.L. ^{[1
]}

Buonaguro F.M. ^{[1
]}

机构：

[1] Laboratory of Molecular Biology and Viral Oncogenesis, AIDS Reference Center, Istituto Nazionale Tumori Fondazione Giovanni Pascale, Naples

[2] Section of Microbiology, Department of Hematology, Oncologic Science, Anatomical Pathology and Microbiology, University of Bologna, Bologna

[3] Interuniversity Consortium, National Institute Biostructure and Biosystem (INBB), Rome

[4] Department of Clinical Medicine, Nephrology, and Health Sciences, University of Parma, Parma

[5] Department of Clinical Sciences, Infectious Diseases Unit 'L. Sacco', University of Milano

[6] VII Division of Infectious Diseases, Cotugno Hospital, Naples

来源：

Infectious Agents and Cancer | / 3卷 / 1期

关键词：

Nest Polymerase Chain Reaction; Sample PR06; Nest Polymerase Chain Reaction Analysis;

D O I：

10.1186/1750-9378-3-13

中图分类号：

学科分类号：

摘要：

Objective. The continuous identification of HIV-1 non-B subtypes and recombinant forms in Italy indicates the need of constant molecular epidemiology survey of genetic forms circulating and transmitted in the resident population. Methods. The distribution of HIV-1 subtypes has been evaluated in 25 seropositive individuals residing in Italy, most of whom were infected through a sexual route during the 1995-2005 period. Each sample has been characterized by detailed molecular and phylogenetic analyses. Results. 18 of the 25 samples were positive at HIV-1 PCR amplification. Three samples showed a nucleotide divergence compatible with a non-B subtype classification. The phylogenetic analysis, performed on both HIV-1 env and gag regions, confirms the molecular sub-typing prediction, given that 1 sample falls into the C subtype and 2 into the G subtype. The B subtype isolates show high levels of intra-subtype nucleotide divergence, compatible with a long-lasting epidemic and a progressive HIV-1 molecular diversification. Conclusion. The Italian HIV-1 epidemic is still mostly attributable to the B subtype, regardless the transmission route, which shows an increasing nucleotide heterogeneity. Heterosexual transmission and the interracial blending, however, are slowly introducing novel HIV-1 subtypes. Therefore, a molecular monitoring is needed to follow the constant evolution of the HIV-1 epidemic. © 2008 Buonaguro et al; licensee BioMed Central Ltd.

引用

共 37 条

[1]

Buonaguro L., Tornesello M.L., Buonaguro F.M., Human immunodeficiency virus type 1 subtype distribution in the worldwide epidemic: Pathogenetic and therapeutic implications, J Virol, 81, pp. 10209-10219, (2007)

[2]

Aids Operative Center I., Aggiornamento dei casi di AIDS notificati Italia - Dec 2006, Not Ist Sup Sanità, 20, S1, (2007)

[3]

Nicolosi A., Molinari S., Musicco M., Saracco A., Ziliani N., Lazzarin A., Positive modification of injecting behavior among intravenous heroin users from Milan and Northern Italy: 1987-1989, Br J Addiction, 86, pp. 91-102, (1991)

[4]

Rezza G., De Rose A., Dorrucci M., Arpino C., Serafin I., Declining prevalence of HIV infection among injecting drug users entering drug treatment in Italy: 1990-1991, Eur J Epidemiol, 9, pp. 663-666, (1993)

[5]

Balotta C., Violin M., Monno L., Bagnarelli P., Riva C., Facchi G., Berlusconi A., Lippi M., Rusconi S., Clementi M., Galli M., Angarano G., Prevalence of multiple dideoxynucleoside analogue resistance (MddNR) in a multicenter cohort of HIV-1-infected Italian patients with virologic failure, J Acquir Immune Defic Syndr, 24, 3, pp. 232-240, (2000)

[6]

Balotta C., Facchi G., Violin M., Van Dooren S., Cozzi-Lepri A., Forbici F., Bertoli A., Riva C., Senese D., Caramello P., Carnevale G., Rizzardini G., Cremonini L., Monno L., Rezza G., Perno C.F., Ippolito G., D'Arminio-Monforte A., Vandamme A.M., Moroni M., Increasing prevalence of non-clade B HIV-1 strains in heterosexual men and women, as monitored by analysis of reverse transcriptase and protease sequences, J Acquir Immune Defic Syndr, 27, 5, pp. 499-505, (2001)

[7]

Ceccherini-Silberstein F., Erba F., Gago F., Bertoli A., Forbici F., Bellocchi M.C., Gori C., D'Arrigo R., Marcon L., Balotta C., Antinori A., Monforte A.D., Perno C.F., Identification of the minimal conserved structure of HIV-1 protease in the presence and absence of drug pressure, AIDS, 18, (2004)

[8]

Monno L., Brindicci G., Lo C.S., Punzi G., Scarabaggio T., Riva C., Di B.C., Pierotti P., Saracino A., Lagioia A., Mazzotta F., Balotta C., Angarano G., HIV-1 subtypes and circulating recombinant forms (CRFs) from HIV-infected patients residing in two regions of central and southern Italy, J Med Virol, 75, pp. 483-490, (2005)

[9]

Venturi G., Romano L., Carli T., Corsi P., Pippi L., Valensin P.E., Zazzi M., Divergent distribution of HIV-1 drug-resistant variants on and off antiretroviral therapy, Antivir Ther, 7, 4, pp. 245-250, (2002)

[10]

Bagnarelli P., Vecchi M., Burighel N., Bellanova D., Menzo S., Clementi M., De R.A., Genotypic and phenotypic correlates of the HIV Type 1 env gene evolution in infected children with discordant response to antiretroviral therapy, AIDS Res Hum Retroviruses, 20, pp. 1306-1313, (2004)

← 1 2 3 4 →