Protective Effects of Piperine Against Corticosterone-Induced Neurotoxicity in PC12 Cells

被引：0
作者：
Qing-Qiu Mao
Zhen Huang
Siu-Po Ip
Yan-Fang Xian
Chun-Tao Che
机构：
[1] The Chinese University of Hong Kong,School of Chinese Medicine
[2] Zhejiang Chinese Medicine University,College of Pharmacy
[3] University of Illinois at Chicago,Department of Medicinal Chemistry and Pharmacognosy
来源：
Cellular and Molecular Neurobiology | 2012年 / 32卷
关键词：
Piperine; Antidepressant; Corticosterone; PC12 cells; Neuroprotection;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Hyperactivation of the hypothalamic–pituitary–adrenal axis and the associated hippocampal atrophy were observed in patients with depression, which could be ameliorated by the treatment with antidepressants. Therefore, neuroprotection has been proposed to be one of the acting mechanisms of antidepressant. Our previous studies have showed that treating mice with piperine produced antidepressant-like effect in animal models of behavioral despair. This study aimed to examine the protective effect of piperine treatment on corticosterone-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The results showed that piperine co-treatment revealed a differential effect on the cytotoxicity of corticosterone and had its maximum inhibitory effect at 1 μM. Piperine (1 μM) co-treatment also significantly decreased intracellular reactive oxygen species level, and enhanced superoxide dismutase activity and total glutathione level in corticosterone-treated PC12 cells. In addition, piperine (1 μM) co-treatment was found to reverse the decreased brain-derived neurotrophic factor (BDNF) mRNA level caused by corticosterone in PC12 cells. The results suggest that piperine exerts a neuroprotective effect on corticosterone-induced neurotoxicity in PC12 cells, at least in part, via the inhibition of oxidative stress and the upregulation of BDNF mRNA expression. This neuroprotective effect may be one of the acting mechanisms accounts for the in vivo antidepressant activity of piperine.
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页码：531 / 537
页数：6
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