Monolayers assembled from a glycolipid biosurfactant from Pseudozyma (Candida) antarctica serve as a high-affinity ligand system for immunoglobulin G and M

被引：0
作者：
Tomohiro Imura
Seya Ito
Reiko Azumi
Hiroshi Yanagishita
Hideki Sakai
Masahiko Abe
Dai Kitamoto
机构：
[1] National Institute of Advanced Industrial Science and Technology (AIST),Research Institute for Innovation in Sustainable Chemistry
[2] Tokyo University of Science,Faculty of Science and Technology
[3] National Institute of Advanced Industrial Science and Technology (AIST),Photonics Research Institute
来源：
Biotechnology Letters | 2007年 / 29卷
关键词：
Atomic force microscopy; Biosurfactant; Immunoglobulin; Protein-carbohydrate interaction; Surface plasmon resonance;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A carbohydrate ligand system has been developed which is composed of self-assembled monolayers (SAMs) of mannosylerythritol lipid-A (MEL-A) from Pseudozyma antarctica, serving for human immunoglobulin G and M (HIgG and HIgM). The estimated binding constants from surface plasmon resonance (SPR) measurement were Ka = 9.4 × 106 M−1 for HIgG and 5.4 × 106 M−1 for HIgM, respectively. The binding site was not in the Fc region of immunoglobulin but in the Fab region. Large amounts of HIgG and HIgM bound to MEL-A SAMs were directly observed by atomic force microscopy.
引用
收藏
页码：865 / 870
页数：5
相关论文
共 90 条
[1]  
Akerstrom B(1989)Protein L: an immunoglobulin light chain-binding bacterial protein J Biol Chem 264 19740-19746
[2]  
Bjorck L(2000)B-1 and B-2 Cell-derived immunoglobulin M antibodies are nonredundant components of the protective response to influenza virus infection J Exp Med 192 271-280
[3]  
Baumgarth N(1991)Crystallization and preliminary X-ray diffraction study of a chimaeric Fab′ fragment of antibody binding tumour cells J Mol Biol 229 603-604
[4]  
Herman OC(1997)Nanometer-scale surface properties of mixed phospholipid monolayers and bilayers Langmuir 13 4779-4784
[5]  
Jager CC(2003)Mannosylerythritol lipids, yeast glycolipid biosurfactants, are potential affinity ligand materials for human immunoglobulins G J Biomed Mater Res 65 379-385
[6]  
Brown LE(2006)Naturally engineered glycolipid biosurfactants leading to distinctive self-assembled structures Chem Eur J 12 2434-2440
[7]  
Herzenberg LA(2005)Thermodynamically stable vesicle formation from glycolipid biosurfactant sponge phase Colloids Surf B 43 115-121
[8]  
Chen J(2004)Coacervate formation from natural glycolipid: one acetyl group on the headgroup triggers coacervate-to-vesicle transition J Am Chem Soc 126 10804-10805
[9]  
Brady RL(2002)Functions and potential applications of glycolipid biosurfactants-from energy-saving materials to gene delivery carriers J Biosci Bioeng 94 187-201
[10]  
Hubbard RE(1998)Polyvalent interactions in biological system: implications for design and use of multivalent ligands and inhibitors Angew Chem Int Ed 37 2754-2794
123456789