Large scale purification protocol for carnocin KZ 213 from Carnobacterium piscicola

被引：8

作者：

Saint-Hubert C. ^{[1
]}

Durieux A. ^{[1
]}

Bodo E. ^{[2
]}

Simon J.-P. ^{[3
]}

机构：

[1] Institut Meurice, Unité de BioTechnologie, Campus CERIA, Brussels 1070

[2] Meurice R and D Asbl, Brussels 1070

[3] Eurobiotec Sa, Brussels 1070

来源：

Biotechnology Letters | 2009年 / 31卷 / 04期

关键词：

Bacteriocin; Carnobacterium; Lactic acid bacteria; Protein purification;

D O I：

10.1007/s10529-008-9897-9

中图分类号：

学科分类号：

摘要：

Large scale purification of a class IIa bacteriocin has been developed to recover pure carnocin KZ 213 produced by Carnobacterium piscicola 213. Most previous protocols reported in the literature for the purification of small peptides have used reversed phase chromatography but scale-up is difficult. The first step of this new protocol is hydrophobic interaction chromatography, the second and third steps are cation exchange chromatography. The protocol leads to a complete recovery of carnocin KZ 213 with 95% purity and to a concentration factor of 83. From 10 l culture supernatant, 5.8 mg carnocin KZ 213 have been produced with a specific activity of 8,500 UA g-1. The protocol is easy to implement for larger volumes. © 2008 Springer Science+Business Media B.V.

引用

页码：519 / 523

页数：4

共 11 条

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