Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.)

被引：26

作者：

Al-Khayri J.M. ^{[1
]}

机构：

[1] Date Palm Research Center, Coll. of Agriculture and Food Sci., King Faisal University, Al-Hassa 31982

来源：

In Vitro Cellular & Developmental Biology - Plant | 2001年 / 37卷 / 4期

关键词：

Callus; Regeneration; Somatic embryogenesis; Tissue culture; Vitamins;

D O I：

10.1007/s11627-001-0079-x

中图分类号：

学科分类号：

摘要：

This study was conducted to examine the effect of biotin and thiamine concentrations on callus growth and somatic embryogenesis of date palm (Phoenix dactylifera L.). Embryogenic callus derived from offshoot tip explants was cultured on hormone-free MS medium containing biotin at 0, 0.1, 1, or 2 mg l-1 combined with thiamine at 0.1, 0.5, 2, or 5 mg l-1. Embryogenic callus weight, number of resultant embryos, and embryo length were significantly influenced by thiamine and biotin concentration. The optimum callus growth treatment consisted of 0.5 mg l-1 thiamine and 2 mg l-1 biotin. This treatment also gave the highest number of embryos. Embryo elongation was greatest at 0.5 or 2 mg l-1 thiamine combined with 1 mg l-1 biotin. Embryos from all treatments germinated and regenerants exhibited normal growth in soil. This study provides an insight into the importance of optimizing various culture medium components to overcome in vitro recalcitrance of date palm.

引用

页码：453 / 456

页数：3

共 21 条

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