Deuterium isotope effects and fractionation factors of hydrogen-bonded A:T base pairs of DNA

被引：0
作者：
Ioannis Vakonakis
Miguel Salazar
Mijeong Kang
Kim R. Dunbar
Andy C. LiWang
机构：
[1] Texas A&M University,Departments of Biochemistry & Biophysics
[2] University of Texas,undefined
[3] College of Pharmacy,undefined
来源：
Journal of Biomolecular NMR | 2003年 / 25卷
关键词：
chemical shift; DNA; deuterium isotope effect; fractionation factors; hydrogen bond; imino; Watson–Crick;
D O I：
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中图分类号：
学科分类号：
摘要：
Deuterium isotope effects and fractionation factors of N1...H3–N3 hydrogen bonded Watson–Crick A:T base pairs of two DNA dodecamers are presented here. Specifically, two-bond deuterium isotope effects on the chemical shifts of 13C2 and 13C4, 2Δ13C2 and 2Δ13C4, and equilibrium deuterium/protium fractionation factors of H3, Φ, were measured and seen to correlate with the chemical shift of the corresponding imino proton, δH3. Downfield-shifted imino protons associated with larger values of 2Δ13C2 and 2Δ13C4 and smaller Φ values, which together suggested that the effective H3–N3 vibrational potentials were more anharmonic in the stronger hydrogen bonds of these DNA molecules. We anticipate that 2Δ13C2, 2Δ13C4 and Φ values can be useful gauges of hydrogen bond strength of A:T base pairs.
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页码：105 / 112
页数：7
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