Establishment of Physalis minima hairy roots culture for the production of physalins

被引：22

作者：

Jualang Azlan G. ^{[1
]}

Marziah M. ^{[2
]}

Radzali M. ^{[2
]}

Johari R. ^{[2
]}

机构：

[1] School of Science and Technology, Universiti Malaysia Sabah, Kota Kinabalu, Sabah 88999

[2] Department of Biochemistry and Microbiology, Universiti Putra Malaysia, Serdang

来源：

Plant Cell, Tissue and Organ Culture | 2002年 / 69卷 / 3期

关键词：

Agrobacterium rhizogenes; Genetic transformation; Physalins production; Physalis minima; Transformed roots;

D O I：

10.1023/A:1015662118877

中图分类号：

学科分类号：

摘要：

This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were investigated in various basal media grown under dark and light conditions, and compared to that of normal root cultures. Transformed hairy root cultures grew rapidly and reach stationary phase after 15 days on a B5 medium. HPLC analysis of extracts of hairy root cultures showed that the maximum content of physalin B and F was 1.82 and 4.15 mg g-1 DW, respectively, when grown under dark conditions. Normal root cultures produced higher physalin B (1.60-1.62 mg g-1 DW) and F (3.30-3.75 mg g-1 DW) under the same culture conditions. Physalin F synthesis in light-grown root cultures was reduced significantly.

引用

页码：271 / 278

页数：7

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