Detection of Streptococcus pneumoniae from culture-negative dried blood spots by real-time PCR in Nigerian children with acute febrile illness

被引：9

作者：

Iroh Tam P.-Y. ^{[1
,8
,9
]}

Hernandez-Alvarado N. ^{[1
]}

Schleiss M.R. ^{[1
]}

Yi A.J. ^{[1
]}

Hassan-Hanga F. ^{[2
]}

Onuchukwu C. ^{[3
]}

Umoru D. ^{[4
]}

Obaro S.K. ^{[2
,5
,6
,7
]}

机构：

[1] Division of Pediatric Infectious Diseases and Immunology, University of Minnesota, Minneapolis, MN

[2] Aminu Kano Teaching Hospital, Kano

[3] Federal Medical Center, Keffi, Nasarawa

[4] Nyanya General Hospital, Abuja

[5] University of Nebraska Medical Center, Omaha, NE

[6] International Foundation Against Infectious Diseases in Nigeria, Abuja

[7] University of Abuja Teaching Hospital, Gwagwalada

[8] Malawi-Liverpool Wellcome Trust Clinical Research Programme, Blantyre

[9] Liverpool School of Tropical Medicine, Liverpool

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Bloodstream infections; Children; Dried blood spot; Febrile illness; Molecular diagnostics; Pneumococcus;

D O I：

10.1186/s13104-018-3770-2

中图分类号：

学科分类号：

摘要：

Objectives: Acute febrile illness is a common cause of hospital admission, and its associated infectious causes, of which a key bacterial causative agent is Streptococcus pneumoniae, contribute to substantial morbidity and mortality. We sought to evaluate the utility of real-time (rt)-PCR on dried blood spots (DBS) for diagnosis of S. pneumoniae in acute febrile illness among children presenting to hospitals in Nigeria. We previously described preliminary results in a sample of 537 patients. Here we present data from a larger collection of 1038 patients. Results: Using rt-PCR for Streptococcus pneumoniae on 1038 dried blood spots from children prospectively enrolled with acute febrile illness, including 79 healthy controls, we detected pneumococcal DNA in nine of 15 blood culture-positive specimens, one culture-negative specimen from a high-risk group, a culture-confirmed non-pneumococcal specimen and a healthy control. Six culture-positive isolates (40%) were negative. Sensitivity was 60%, specificity 99.7%, positive predictive value 75% and negative predictive value 99.4%. Rt-PCR of DBS has limited sensitivity in blood specimens from acute febrile illness in children. © 2018 The Author(s).

引用

共 8 条

[1] Iroh Tam P.Y., Thielen B.K., Obaro S.K., Et al., Childhood pneumococcal disease in Africa - A systematic review and meta-analysis of incidence, serotype distribution, and antimicrobial susceptibility, Vaccine, 35, pp. 1817-1827, (2017)
[2] Iroh Tam P.Y., Hernandez-Alvarado N., Schleiss M.R., Et al., Molecular detection of Streptococcus pneumoniae on dried blood spots from febrile Nigerian children compared to culture, PLoS ONE, 11, (2016)
[3] Obaro S., Lawson L., Essen U., Et al., Community acquired bacteremia in young children from central Nigeria - A pilot study, BMC Infect Dis, 11, (2011)
[4] Morpeth S.C., Deloria Knoll M., Scott J.A.G., Et al., Detection of pneumococcal DNA in blood by polymerase chain reaction for diagnosing pneumococcal pneumonia in young children from low- and middle-income countries, Clin Infect Dis, 64, pp. S347-S356, (2017)
[5] Adetifa I.M., Antonio M., Okoromah C.A., Et al., Pre-vaccination nasopharyngeal pneumococcal carriage in a Nigerian population: Epidemiology and population biology, PLoS ONE, 7, (2012)
[6] Selva L., Benmessaoud R., Lanaspa M., Et al., Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: A useful approach for developing countries, PLoS ONE, 8, (2013)
[7] Hwang J., Jaroensuk J., Leimanis M.L., Et al., Long-term storage limits PCR-based analyses of malaria parasites in archival dried blood spots, Malar J, 11, (2012)
[8] Schwartz A., Baidjoe A., Rosenthal P.J., Dorsey G., Bousema T., Greenhouse B., The effect of storage and extraction methods on amplification of Plasmodium falciparum DNA from dried blood spots, Am J Trop Med Hyg, 92, pp. 922-925, (2015)

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