Acteoside inhibits irradiation-mediated decreases in the viability and DNA synthesis of MC3T3-E1 cells

被引：0
作者：
Kyoung-A. Kim
Seung-Ah Lee
Ki-Hyun Kim
Keun-Soo Lee
Jeong-Chae Lee
机构：
[1] Chonbuk National University,Department of Oral and Maxillofacial Radiology and School of Dentistry, Research Institute of Clinical Medicine
[2] Chonnam Techno College,Department of Nursing
[3] Chonbuk National University,Department of Orthodontics and School of Dentistry
[4] Korea Bone Bank Co.,Research Laboratory, RM317 Acetechno Tower
[5] Ltd.,9
[6] Chonbuk National University,Research Center of Bioactive Materials
来源：
Food Science and Biotechnology | 2013年 / 22卷
关键词：
X-ray radiation; MC3T3-E1 cell; oxidative damage; acteoside; viability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Therapeutic irradiation can cause bone loss, whereas antioxidant supplementation is considered to attenuate irradiation-mediated damages. This study examined whether or not acteoside inhibits irradiation-mediated changes in viability and proliferation of MC3T3-E1 cells. X-ray radiation at >4 Gy not only decreased cell viability and DNA synthesis in the cells, but also increased intracellular levels of reactive oxygen species (ROS) and phosphorylated p66Shc protein. Irradiation at 8Gy also decreased intracellular levels of reduced glutathione (GSH) and induced G1 phase arrest of cell cycle progression with the attendant increase of p21 induction. Pretreatment with acteoside inhibited the irradiation-mediated decreases in viability and DNA synthesis by restoring the radiation-mediated changes in the levels of ROS, GSH, p21, and p-p66Shc to the untreated control levels. These inhibitory activities of acteoside were greater than that of a synthetic antioxidant compound or N-acetyl cysteine did. Collectively, acteoside treatment may prevent irradiation-induced oxidative damages to osteoblasts.
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页码：845 / 851
页数：6
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  • [1] Cohen LE(2012)Bone density in post-pubertal adolescent survivors of childhood brain tumors Pediatr. Blood Cancer 58 959-963
  • [2] Gordon JH(2010)Osteoradionecrosis of the upper cervical spine: MR imaging following radiotherapy for nasopharyngeal carcinoma Eur. J. Radiol. 73 629-635
  • [3] Popovsky EY(2011)Osteoradionecrosis Oral Maxillofac. Surg. Clin. North Am. 23 455-464
  • [4] Sainath NN(2005)Expression of bone morphogenic protein 2/4, transforming growth factor-β1, and bone matrix protein expression in healing area between vascular tibia grafts and irradiated bone-experimental model of osteonecrosis Int. J. Radiat. Oncol. 61 1189-1196
  • [5] Feldman HA(1996)Effect of X-ray irradiation on proliferation and differentiation of osteoblast Calcified Tissue Int. 59 307-308
  • [6] Kieran MW(1997)Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells J. Dent. Res. 76 658-664
  • [7] Gordon CM(2000)The effects of ionizing radiation on osteoblast-like cells Plast. Reconstr. Surg. 106 1049-1061
  • [8] King AD(2004)Ionizing radiation sensitizes bone cells to apoptosis Bone 34 148-156
  • [9] Griffith JF(2011)Effects of irradiation on growth and differentiation-related gene expression in osteoblasts J. Craniofac. Surg. 22 1635-1640
  • [10] Abrigo JM(2001)X-ray irradiation stimulates the mitochondrial-dependent generation of reactive oxygen and nitrogen species in K562 leukemia cells Radiats. Biol. Radioecol. 51 677-683
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