Spectrophotometric determination of procaine hydrochloride with hemoglobin as catalyst

A highly sensitive and simple spectrophotometric method for the determination of procaine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2O2 and acid chrome blue K(ACBK) was developed. The concentration of procaine hydrochloride is in linear relationship with the percentage inhibition (I%) of system under the optimal experimental conditions. The calibration graph is linear in the range from 1.50 × 10−7 to 4.15 × 10−6 M with the detection limit of 3.80 × 10−8 M. This method can be used for the determination of procaine hydrochloride in injection solution of procaine hydrochloride with satisfactory results. The article is published in the original.