The Gibbs Energies of Activation of Lysozyme for Viscous Flow in Water + Dimethyl Sulfoxide Solutions at 298.15 K

被引：0
作者：
Tadashi Kamiyama
Masatsugu Morita
Takayoshi Kimura
机构：
[1] Kinki University,Department of Chemistry, School of Science and Engineering
来源：
Journal of Solution Chemistry | 2008年 / 37卷
关键词：
Gibbs energy of activation for viscous flow; Lysozyme; Dimethyl sulfoxide; Partial specific volume; Intrinsic viscosity;
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摘要：
Gibbs energies of activation for viscous flow of binary water (1) + dimethyl sulfoxide (2) mixtures, Δμ12○, and of lysozyme (3) in corresponding ternary mixtures, Δμ3○, were determined at 298.15 K. The binary mixtures have a maximum in the value of the excess quantity for Δμ12○ at a dimethyl sulfoxide mole fraction of x2≈0.31. The values of Δμ3○ are larger than Δμ12○ at all values of x2, even when normalized by their molar volumes, suggesting that the solvents interact more strongly with lysozyme than with themselves. The values of Δμ3○ significantly increased in the range of x2=0.3 to 0.4 because of an increase in solvent-lysozyme interactions, which resulted from an increase in the accessible surface area of lysozyme that was exposed by its unfolding. The mean value obtained for Δμ3○ per amino acid of lysozyme at x2=0.2 is greater than that for hydrophobic amino acids, indicating that the solvent interacts with hydrophilic amino acids more strongly than with hydrophobic ones.
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页码：27 / 34
页数：7
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共 55 条
  • [11] Sighinolfi S.(1966)Association in the binary liquid system dimethyl sulfoxide-water J. Pharm. Sci. 55 851-853
  • [12] Tassi L.(2003)Freezing point curve of dimethyl sulfoxide-water solutions J. Chem. Eng. Data 48 1301-1305
  • [13] Ulrici A.(2004)Volumetric study of lysozyme in dimethyl sulfoxide + water solution at 298.15 K J. Chem. Eng. Data 49 1350-1353
  • [14] Vignali M.(1992)Rheological study of lysozyme in dimethyl sulfoxide + water solution at 298.15 K Nature 358 302-307
  • [15] Zucchi C.(1976)The folding of hen lysozyme involves partially structured intermediates and multiple pathways Biophys. Chem. 4 23-32
  • [16] Banipal P.K.(1929)Thermodynamic investigations of proteins. I. Standard functions for proteins with lysozyme as an example J. Am. Chem. Soc. 51 1073-1091
  • [17] Gautam S.(1997)The transference number of barium chloride as a function of the concentration Ind. J. Chem. 36A 834-843
  • [18] Dua S.(1998)Partial molar volume, ultrasonic and viscometric studies of glycine, Asian J. Chem. 10 17-22
  • [19] Banipal T.S.(1995)-alanine and J. Mol. Biol. 250 291-306
  • [20] Yan Z.(1995)-alanine in water + DMSO mixtures at different temperatures Biochemistry 34 1878-1884
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