Purification and analysis of DNA from low DNA content food samples

被引：0
作者：
M. Esra Altuntop Yayla
Canan Ekinci Doğan
Şerife Sarioğlan
机构：
[1] TUBITAK MRC,
[2] Food Institute,undefined
[3] TUBITAK MRC,undefined
[4] Chemistry Institute,undefined
来源：
Journal of Consumer Protection and Food Safety | 2019年 / 14卷
关键词：
DNA extraction; Zeolite Y; Low DNA content food; Real-time PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples.
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页码：147 / 155
页数：8
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