Progress and potential in organoid research

被引:0
作者
Giuliana Rossi
Andrea Manfrin
Matthias P. Lutolf
机构
[1] École Polytechnique Fédérale de Lausanne (EPFL),Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering
[2] École Polytechnique Fédérale de Lausanne (EPFL),Institute of Chemical Sciences and Engineering, School of Basic Science
来源
Nature Reviews Genetics | 2018年 / 19卷
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摘要
Tissue and organ biology are very challenging to study in mammals, and progress can be hindered, particularly in humans, by sample accessibility and ethical concerns. However, advances in stem cell culture have made it possible to derive in vitro 3D tissues called organoids, which capture some of the key multicellular, anatomical and even functional hallmarks of real organs at the micrometre to millimetre scale. Recent studies have demonstrated that organoids can be used to model organ development and disease and have a wide range of applications in basic research, drug discovery and regenerative medicine. Researchers are now beginning to take inspiration from other fields, such as bioengineering, to generate organoids that are more physiologically relevant and more amenable to real-life applications.
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页码:671 / 687
页数:16
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[1]  
Murry CE(2008)Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development Cell 132 661-680
[2]  
Keller G(2016)Modeling development and disease with organoids Cell 165 1586-1597
[3]  
Clevers H(2014)Organogenesis in a dish: modeling development and disease using organoid technologies Science 345 1247125-326
[4]  
Lancaster MA(2013)Cytosystems dynamics in self-organization of tissue architecture Nature 493 318-343
[5]  
Knoblich JA(1975)Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells Cell 6 331-1403
[6]  
Sasai Y(1956)The growth of cells on a transparent gel of reconstituted rat-tail collagen J. Natl Cancer Inst. 16 1375-220
[7]  
Rheinwald JG(1977)A murine tumor producing a matrix of basement membrane J. Exp. Med. 145 204-128
[8]  
Green H(1907)Observations of the living developing nerve fiber Anat. Rec. 1 116-78
[9]  
Ehrmann RL(1975)Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations Exp. Cell Res. 94 70-235
[10]  
Gey GO(1989)Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane Development 105 223-9068