Indirect shoot organogenesis from leaves of Dieffenbachia cv. Camouflage

被引：0
作者：
Xiuli Shen
Jianjun Chen
Michael E. Kane
机构：
[1] University of Florida,Department of Environmental Horticulture, Institute of Food and Agricultural Sciences
[2] University of Florida,Mid
来源：
Plant Cell, Tissue and Organ Culture | 2007年 / 89卷
关键词：
Ornamental foliage plants; Shoot organogenesis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel protocol for indirect shoot organogenesis of Dieffenbachia cv. Camouflage was established using leaf explants excised from in vitro shoot cultures. The frequency of callus formation reached 96% for explants cultured on Murashige and Skoog (1962) basal medium supplemented with 5 μM thidiazuron and 1 μM 2,4-dichlorophenozyacetic acid. The number of shoots regenerated was high, with up to 7.9 shoots produced per callus cultured on basal medium supplemented with 40 μM N6-(Δ2-isopentenyl)adenine and 2 μM indole-3-acetic acid. Regenerated shoots rooted well in a soilless substrate, acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse conditions. Somaclonal variations in leaf variegation, color, and morphology have been observed in regenerated plants.
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页码：83 / 90
页数：7
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