Effects of Zn2+ and Cu2+ on growth, lignin degradation and ligninolytic enzymes in Phanerochaete chrysosporium

被引：0
作者：
Vikas Singhal
V.S. Rathore
机构：
[1] Govind Ballabh Pant University of Agriculture and Technology,Department of Environmental Sciences, College of Basic Sciences and Humanities
[2] Pantnagar –,undefined
来源：
World Journal of Microbiology and Biotechnology | 2001年 / 17卷
关键词：
Lignin biodegradation; ligninolytic enzymes; lignin peroxidase; manganese peroxidase; trace elements;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The influence of Zn2+ (6.0 × 10−3 –18.0 × 10−3μM) and Cu2+ (4 × 10−4 –1.2 × 10−4μM) in the basal medium on mycelial growth (dry weight), activities of lignin peroxidase (Lip), manganese peroxidase (Mnp), solubilization, and mineralization (14CO2 evolution) of lignin during a period of 3 weeks was studied in Phanerochaete chrysosporium strain MTCC-787. Highest mycelial growth was obtained at 0.6 μM Zn2+ and 0.4 μM Cu2+ levels. Enzyme activities were found to increase up to the highest levels of both the trace elements. However, Zn2+ had a relatively more stimulatory effect on Lip production and the reverse was true in case of Cu2+. [14C]Lignin solubilization was also promoted by higher levels of both trace elements. Mineralization of [14C]lignin was optimal at 6.0 μM Zn2+ and 1.2 μM Cu2+. The stimulatory effect of Zn2+ on Lip production was correlated with higher rates of [14C]lignin mineralization.
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页码：235 / 240
页数：5
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