Haplotype-resolved chromosomal-level assembly of wasabi (Eutrema japonicum) genome

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作者：
Hiroyuki Tanaka
Tatsuki Hori
Shohei Yamamoto
Atsushi Toyoda
Kentaro Yano
Kyoko Yamane
Takehiko Itoh
机构：
[1] Tokyo Institute of Technology,School of Life Science and Technology
[2] Meguro-ku,Comparative Genomics Laboratory
[3] Gifu University,Department of Biological Sciences
[4] Faculty of Applied Biological Sciences,undefined
[5] National Institute of Genetics,undefined
[6] Mishima,undefined
[7] Tokyo Metropolitan University,undefined
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Scientific Data | / 10卷
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摘要：
In Japan, wasabi (Eutrema japonicum) is an important traditional condiment, and is recognized as an endemic species. In the present study, we generated a chromosome-level and haplotype-resolved reference genome for E. japonicum using PacBio CLR (continuous long reads), Illumina, and Hi-C sequencing data. The genome consists of 28 chromosomes that contain 1,512.1 Mb of sequence data, with a scaffold N50 length of 55.67 Mb. We also reported the subgenome and haplotype assignment of the 28 chromosomes by read-mapping and phylogenic analysis. Three validation methods (Benchmarking Universal Single-Copy Orthologs, Merqury, and Inspector) indicated that our obtained genome sequences were a high-quality and high-completeness genome assembly. Comparison of genome assemblies from previously published genomes showed that our obtained genome was of higher quality. Therefore, our genome will serve as a valuable genetic resource for both chemical ecology and evolution research of the genera Eutrema and Brassicaceae, as well as for wasabi breeding.
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