Secretory Expression and Characterization of Chinese Narcissus GNA-Like Lectin in Pichia pastoris

被引：0
作者：
Xinyu Li
Pengpeng Zhang
Ning Zhang
Xiao Liang
Wenya Wang
Qipeng Yuan
Qiang Li
机构：
[1] Beijing University of Chemical Technology,College of Life Science and Technology
[2] Tsinghua University,Department of Chemical Engineering
来源：
Applied Biochemistry and Biotechnology | 2017年 / 182卷
关键词：
Chinese ; Mannose-binding lectin; Secretory expression; Metal ions;
D O I：
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中图分类号：
学科分类号：
摘要：
Narcissus tazetta lectin (NTL) is a GNA-like lectin, which has a wide potential application in medicine, agriculture, and glycobiology. In the present paper, the codon-optimized ntl gene was transformed into the yeast Pichia pastoris; SDS–PAGE gel and western blotting analysis revealed that the recombinant lectin was expressed successfully in Pichia yeast. The similarity between the recombinant NTL and the native NTL was confirmed by circular dichroism (CD) and hemagglutination assay further. In the 5-L scale fermentator, the protein yield was as high as 1.2 g/L after fermentation for 96 h. In addition, the effect of metal ions (K+, Mg2+, Ca2+, and Cu2+), acid, and alkaline on hemagglutinating activity of NTL was tested, which provided biochemical characterizations of the mannose-binding lectin from Chinese Narcissus.
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页码：1433 / 1443
页数：10
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