Isolation and Characterization of Ornithine Decarboxylase Gene from Flounder (Paralichthys olivaceus)

被引：0
作者：
Jae Hyung Lee
Mi Young Son
Moon-Young Yoon
Jung-Do Choi
Young Tae Kim
机构：
[1] Pukyong National University,Department of Microbiology
[2] Hanyang University,Department of Chemistry
[3] Chungbuk National University,Department of Life Sciences
来源：
Marine Biotechnology | 2004年 / 6卷
关键词：
ornithine decarboxylase (ODC); pyridoxal 5’-phosphate (PLP); flounder (; ).;
D O I：
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摘要：
Ornithine decarboxylase (ODC) is a homodimeric enzyme dependent on pyridoxal 5’-phosphate. We identified a complementary DNA clone corresponding to ODC from the brain of adult flounder (Paralichthys olivaceus). The flounder ODC cDNA consisted of 2939  bp encoding 272 amino acid residues. The flounder ODC showed 80.3% sequence identity to zebrafish and 70.8% to rat at the amino acid level. Comparison of the structure and nucleotide sequence of the ODC genes revealed that the gene is highly conserved in the flounder, zebrafish, and rat. The presence of ODC mRNA species in brain, kidney, liver, and embryo was confirmed using the reverse transcriptase polymerase chain reaction. The recombinant protein of flounder ODC containing a short histidine tag at the carboxyl terminus was overexpressed in Escherichia coli BL21 (DE3) codon plus using an inducible T7 expression system, and was purified by Ni-NTA affinity chromatography.
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页码：453 / 462
页数：9
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