Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE

被引：0
|
作者：
Ryan A Flynn
Qiangfeng Cliff Zhang
Robert C Spitale
Byron Lee
Maxwell R Mumbach
Howard Y Chang
机构：
[1] Center for Personal Dynamic Regulomes,
[2] Stanford University School of Medicine,undefined
来源：
Nature Protocols | 2016年 / 11卷
关键词：
D O I：
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学科分类号：
摘要：
icSHAPE allows transcriptome-wide RNA structure profiling in living cells. It uses a clickable SHAPE reagent to enable biotin-based enrichment of SHAPE-modified RNA, reducing the necessary sequencing depth.
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页码：273 / 290
页数：17
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