DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India

被引：0
作者：
S. S. Gaikwad
H. V. Ghate
S. S. Ghaskadbi
M. S. Patole
Y. S. Shouche
机构：
[1] Modern College,Department of Zoology
[2] University of Pune,Department of Zoology
[3] National Center for Cell Sciences,undefined
来源：
Molecular Biology Reports | 2012年 / 39卷
关键词：
Western Ghats of India; DNA barcoding; Butterflies; Nymphalidae; Cytochrome c oxidase I;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.
引用
收藏
页码：2375 / 2383
页数：8
相关论文
共 161 条
[1]  
Blaxter ML(2004)The promise of DNA taxonomy Philos Trans R Soc Lond Biol Sci 359 669-679
[2]  
Ratnasingham S(2007)BOLD: the barcode of life data system (wwwbarcodinglifeorg) Mol Ecol Notes 7 355-364
[3]  
Hebert PDN(2003)Biological identification through DNA barcodes Proc R Soc Lond B Biol Sci 270 313-322
[4]  
Hebert PDN(2006)DNA barcoding applied to invasive Leafminers (Diptera: Agromyzidae) in the Philippines Ann Entomol Soc Am 99 204-210
[5]  
Cywinska A(2007)DNA barcodes can distinguish species of Indian mosquitoes (Diptera: Culicidae) J Med Entomol 44 1-7
[6]  
Ball SL(2008)DNA barcoding of new World cicada killers (Hymenoptera: Crabronidae) Zootaxa 1713 27-38
[7]  
de Waard JR(2008)Discrimination of Cricotopus species (Diptera: Chironomidae) by DNA barcoding Bull Entomol Res 98 555-563
[8]  
Scheffer SJ(2005)Biological identification of mayflies (Ephemeroptera) using DNA barcodes J North Am Benthol Soc 24 508-524
[9]  
Lewis ML(2004)Biological identification of springtails (Hexapoda: Collembola) from the Canadian arctic using mitochondrial DNA barcodes Can J Zool 82 749-754
[10]  
Joshi RC(2005)DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar Philos Trans R Soc Lond Biol Sci 360 1825-1834
12345678910