Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China

被引:0
作者
Wenguang Xiong
Yongxue Sun
Tong Zhang
Xueyao Ding
Yafei Li
Mianzhi Wang
Zhenling Zeng
机构
[1] South China Agricultural University,National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, College of Veterinary Medicine
[2] The University of Hong Kong,Environmental Biotechnology Laboratory, Department of Civil Engineering
来源
Microbial Ecology | 2015年 / 70卷
关键词
Antibiotic resistance genes; Antibiotics; Bacterial community composition; Pathogens; Fish ponds;
D O I
暂无
中图分类号
学科分类号
摘要
Environmental antibiotic resistance has drawn increasing attention due to its great threat to human health. In this study, we investigated concentrations of antibiotics (tetracyclines, sulfonamides and (fluoro)quinolones) and abundances of antibiotic resistance genes (ARGs), including tetracycline resistance genes, sulfonamide resistance genes, and plasmid-mediated quinolone resistance genes, and analyzed bacterial community composition in aquaculture environment in Guangdong, China. The concentrations of sulfametoxydiazine, sulfamethazine, sulfamethoxazole, oxytetracycline, chlorotetracycline, doxycycline, ciprofloxacin, norfloxacin, and enrofloxacin were as high as 446 μg kg−1 and 98.6 ng L−1 in sediment and water samples, respectively. The relative abundances (ARG copies/16S ribosomal RNA (rRNA) gene copies) of ARGs (sul1, sul2, sul3, tetM, tetO, tetW, tetS, tetQ, tetX, tetB/P, qepA, oqxA, oqxB, aac(6′)-Ib, and qnrS) were as high as 2.8 × 10−2. The dominant phyla were Proteobacteria, Bacteroidetes, and Firmicutes in sediment samples and Proteobacteria, Actinobacteria and Bacteroidetes in water samples. The genera associated with pathogens were also observed, such as Acinetobacter, Arcobacter, and Clostridium. This study comprehensively investigated antibiotics, ARGs, and bacterial community composition in aquaculture environment in China. The results indicated that fish ponds are reservoirs of ARGs and the presence of potential resistant and pathogen-associated taxonomic groups in fish ponds might imply the potential risk to human health.
引用
收藏
页码:425 / 432
页数:7
相关论文
共 256 条
[1]  
Wright GD(2010)Antibiotic resistance in the environment: a link to the clinic? Curr Opin Microbiol 13 589-594
[2]  
Finley RL(2013)The scourge of antibiotic resistance: the important role of the environment Clin Infect Dis 57 704-710
[3]  
Collignon P(2011)Antibiotic resistance is ancient Nature 477 457-461
[4]  
Larsson DJ(2013)Diverse and abundant antibiotic resistance genes in Chinese swine farms Proc Natl Acad Sci U S A 110 3435-3440
[5]  
McEwen SA(2014)Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach Water Res 62 97-106
[6]  
Li X-Z(2014)Antimicrobial resistance and antimicrobial resistance genes in marine bacteria from salmon aquaculture and non‐aquaculture sites Environ Microbiol 16 1310-1320
[7]  
Gaze WH(2014)Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China Environ Int 65 9-15
[8]  
Reid-Smith R(2014)Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes Vet Microbiol 171 441-447
[9]  
Timinouni M(2012)Prevalence of antibiotic resistance genes in the bacterial flora of integrated fish farming environments of Pakistan and Tanzania Environ Sci Technol 46 8672-8679
[10]  
Graham DW(2009)Human health consequences of use of antimicrobial agents in aquaculture Clin Infect Dis 49 1248-1253