Toward a molecular understanding of nanoparticle-protein interactions

被引：124
作者：
Treuel L. [1 ,2 ]
Nienhaus G.U. [1 ,3 ]
机构：
[1] Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT)
[2] Institute of Physical Chemistry, University of Duisburg-Essen
[3] Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL
来源：
Biophysical Reviews | 2012年 / 4卷 / 2期
基金：
日本学术振兴会;
关键词：
Nanoparticle imaging; Nanoparticle spectroscopy; Nanoparticle toxicity; Nanoparticle-protein interactions; Nanoparticles; Protein corona;
D O I：
10.1007/s12551-012-0072-0
中图分类号：
学科分类号：
摘要：
Wherever nanoparticles (NPs) come in contact with a living organism, physical and chemical interactions take place between the surfaces of the NPs and biomatter, in particular proteins. When NP are exposed to biological fluids, an adsorption layer of proteins, a "protein corona" forms around the NPs. Consequently, living systems interact with the protein-coated NP rather than with a bare NP. To anticipate biological responses to NPs, we thus require comprehensive knowledge of the interactions at the bio-nano interface. In recent years, a wide variety of biophysical techniques have been employed to elucidate mechanistic aspects of NP-protein interactions. In this brief review, we present the latest findings regarding the composition of the protein corona as it forms on NPs in the blood stream. We also discuss molecular aspects of this adsorption layer and its time evolution. The current state of knowledge is summarized, and issues that still need to be addressed to further advance our understanding of NP-protein interactions are identified. © 2012 International Union for Pure and Applied Biophysics (IUPAB) and Springer.
引用
收藏
页码：137 / 147
页数：10
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