Expression Analysis of Heat Shock Genes in the Skin, Spleen and Blood of Common Carp (Cyprinus Carpio) after Cadmium Exposure and Hypothermia

被引：0
作者：
Ágnes Ferencz
Renáta Juhász
Monica Butnariu
Aranka K. Deér
Ilona S. Varga
J. Nemcsók
机构：
[1] University of Szeged,Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics
[2] Banatâs University of Agricultural Sciences and Veterinary Medicine from Timisoara,Department of Exact Sciences
[3] J. Selye University,Department of Biology
来源：
Acta Biologica Hungarica | 2012年 / 63卷
关键词：
Cadmium treatment; carp; heat shock genes; RT-PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Heat shock proteins are chaperones that play a pivotal role in controling multiple regulatory pathways such as stress defense, hormone signaling, cell cycle control, cell proliferation and differentiation, and apoptosis. In this study, the expression patterns of four well-known heat shock genes (hsp70, hsc70-1, hsc70-2 and hsp90α) were characterized in the skin, spleen and blood cells of the common carp, under unstressed conditions and after Cd2+ treatment or hypothermia. The examined genes were expressed in a tissue-specific manner: hsc70-2 was expressed constitutively, and was at best only slightly inducible; hsp90α exhibited a high basic expression in all three tissues, whereas hsc70-1 did so only in the blood cells, the expression of hsp70 proved to be below the level of detection in unstressed fish. Cold shock induced the expression of hsp genes in the spleen (hsp90α) and blood cells (hsp70, hsc70-1 and hsp90α), while Cd2+ treatment has no effect on the expression pattern. The highest inducibilities were detected in the skin: for hsp70 an induction of at least 20-fold after cadmium exposure, for hsc70-1 of at least 30-fold and for hsp90α of 3-fold after hypothermia.
引用
收藏
页码：15 / 25
页数：10
相关论文
共 158 条
  • [11] Todgham A E A R A(2007)Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology Aquat. Toxicol. 85 28-39
  • [12] Bibeau M R(2010)Stress proteins induced by exposure to sublethal levels of heavy metals in sea bream Oncol. Rep. 23 1483-1492
  • [13] Nakano K(1996) blood cells Nature 381 571-580
  • [14] Schulte P M(1999)Comparisons of tissue-specific transcription of stress response genes with whole animal endpoints of adverse effect in striped bass Arch. Environ. Contain. Toxicol. 37 78-84
  • [15] Iwama G K(1993) following treatment with copper and esfenvalerate Annu. Rev. Biochem. 62 349-384
  • [16] Csermely P(2001)HSP90 and its inhibitors Comp. Biochem. Physiol. Part C. 129 397-407
  • [17] Schnaider T(1998)Molecular chaperones in cellular protein folding Review, in Fish Biol. Fish 8 35-56
  • [18] Soti C(2006)Induction of Hsp70 by the herbicide oxyfluoren (goal) in the Egyptian Nile fish, Int. J. Cosm. Sci. 28 233-241
  • [19] Prohaszka Z(1998)Molecular chaperone functions of heat-shock proteins Pharmacol. Ther. 80 183-201
  • [20] Nardai G(1994)Identification of two hsp90 genes in carp Biochem. Biophys. Res. Commun. 204 746-752
12345678910