Identification, Characterization and Expression Profiles of Dof Transcription Factors in Pineapple (Ananas comosus L)

被引：0
作者：
Syed Muhammad Azam
Yanhui Liu
Zia Ur Rahman
Hina Ali
Cheng Yan
Lulu Wang
S. V. G. N. Priyadarshani
Binyan Hu
Xinyu Huang
Junjie Xiong
Yuan Qin
机构：
[1] Fujian Agriculture and Forestry University,State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministry of Education, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Haixi
[2] Fujian Agriculture and Forestry University,College of Crop Sciences
[3] Fujian Agriculture and Forestry University,College of Resources and Environment
来源：
Tropical Plant Biology | 2018年 / 11卷
关键词：
Gene expression; Dof transcription factors; Phylogenetic analysis; RNA-Seq; Pineapple;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
DNA binding with one finger (Dof) transcription factors (TF) family is a class of plant specific transcription factors and has crucial roles in development of different tissues and response to stresses. Using GWAS we identified twenty six Dof genes located on 16 pineapple chromosomes. Dof family genes were distributed into five groups and contain highly conserved motif. High expression of AcoDof1, AcoDof12, AcoDof26 (root), AcoDof9, AcoDof22, AcoDof19 (fruit), AcoDof23, AcoDof25 (ovule), AcoDof11, AcoDof17 and AcoDof20 (petal) were found in RNA-Seq analysis. RNA-Seq data also suggest that Dof genes could have important role in female gametophyte development of pineapple. Expression analysis using qRT-PCR of pineapple Dof genes family under different abiotic stress (cold, heat, salt, drought) showed a dynamic response of Dof genes. AcoDof1 was up-regulated at 48 h treatment in all four abiotic stresses, while AcoDof20 was up-regulated at 24 h of treatment. Moreover, AcoDof8, AcoDof12, AcoDof2 were up-regulated by salinity stress. Dof genes expression during abiotic stress reveals their vital role for pineapple growth and development which could be utilized agronomically. Present study provides a framework for future studies related to the functions of Dof genes in pineapple.
引用
收藏
页码：49 / 64
页数：15
相关论文
共 124 条
  • [1] Ali H(2017)Genomic Survey, Characterization, and Expression Profile Analysis of the SBP Genes in Pineapple (Ananas comosus L.) Int J Genomics 2017 1032846-566
  • [2] Liu Y(2013)Genome-wide Analysis of Plant-specific Dof Transcription Factor Family in Tomato J Integr Plant Biol 55 552-1596
  • [3] Azam SM(2017)ERECTA signaling controls Arabidopsis inflorescence architecture through chromatin-mediated activation of PRE1 expression New Phytol 214 1579-1012
  • [4] Cai X(2016)Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance PLoS One 11 e0163315-1292
  • [5] Zhang Y(2017)Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr.) Reveal the Role of AcABCG38 in Pollen Development Front Plant Sci 8 2150-9170
  • [6] Zhang C(2014)Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses J Exp Bot 65 995-84
  • [7] Cai H(2017)H2A.Z Represses Gene Expression by Modulating Promoter Nucleosome Structure and Enhancer Histone Modifications in Arabidopsis Mol Plant 10 1274-3788
  • [8] Zhao L(2014)metaseq: a Python package for integrative genome-wide analysis reveals relationships between chromatin insulators and associated nuclear mRNA Nucleic Acids Res 42 9158-1263
  • [9] Wang L(2007)Wheat Dof transcription factor WPBF interacts with TaQM and activates transcription of an alpha-gliadin gene during wheat seed development Plant Mol Biol 63 73-1026
  • [10] Chen C(2003)ExPASy: the proteomics server for in-depth protein knowledge and analysis Nucleic Acids Res 31 3784-3534
12345678910