AGEs induce epithelial to mesenchymal transformation of human peritoneal mesothelial cells via upregulation of STAT3

被引：0
作者：
Pei Zhang
Hong Dai
Lei Peng
机构：
[1] The First Affiliated Hospital of Anhui Medical University,Department of Nephrology
[2] AnHui Medical University,Institute of pharmacology
来源：
Glycoconjugate Journal | 2019年 / 36卷
关键词：
AGEs; EMT; STAT3; HPMCs; miRNA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Epithelial-mesenchymal transition (EMT) in peritoneum was induced during peritoneal dialysis (PD), which finally caused progressive fibrosis. However, the underlying mechanisms were not well elucidated. We established advanced glycation end-products (AGEs)-induced EMT model using primary human peritoneal mesothelial cells (HPMCs). The working concentration and time of AGEs were optimized. Then the expression and activation signal transducer and activator of transcription 3 (STAT3), a key factor of EMT in cancer, were detected. The regulation of STAT3 by miRNA was also explored. 50 μg/ml AGEs treatment for 24 h can successfully induce EMT in HPMCs. AGEs treatment upregulated and activated STAT3. miRNA-454, potentially targeting STAT3, was down-regulated in AGEs-treated HPMCs. Overexpression of miRNA-454 prevented AGEs- induced EMT in HPMCs. AGEs induce epithelial to mesenchymal transformation of Human peritoneal mesothelial cells via upregulation of STAT3.
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页码：155 / 163
页数：8
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