Difference in expression of Bcl-2 and Bcl-xl genes in cisplatin-sensitive and cisplatin-resistant human in ovarian cancer cell lines

被引：0
作者：
Yu Lili
Wang Zehua
机构：
[1] Huazhong University of Science and Technology,Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College
来源：
Current Medical Science | 2004年 / 24卷 / 2期
关键词：
ovarian cancer; apoptosis; multidrug-resistance; Bcl-2 and Bcl-xl; cisplatin;
D O I：
10.1007/BF02885416
中图分类号：
学科分类号：
摘要：
To investigate the expression of Bcl-2 and Bcl-xl gene in sensitive (A2780) and drug-resistance (AD6) human ovarian cancer cell lines and explore the molecular mechanism of multidrug resistance. A2780 and AD6 were detected by using DNA gel electrophoresis, flow cytometry and RT-PCR. Our results showed that (1) “DNA ladder” was observed in A2780 and AD6 after cisplatin treatment; (2) after 3.0, 6.0, 9.9 μg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between in A2780 and AD6 (P<0.05); (3) Bcl-2 and Bcl-xl genes were overexpressed in AD6. After cisplatin treatment, the expression of Bcl-2 and Bcl-xl genes was down-regulated in A2780 and AD6. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.
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页码：151 / 153
页数：2
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