A novel Lawsonia inermis (Henna)/(hydroxyethylcellulose/polyvinylpyrrolidone) wound dressing hydrogel: radiation synthesis, characterization and biological evaluation

A traditional plant extract from Lawsonia inermis (Henna) with proven antimicrobial properties had been used for the preparation of (hydroxyethylcellulose/polyvinylpyrrolidone) hydrogel dressing using gamma irradiation for crosslinking and sterilization techniques. Henna dressings were characterized by studying the effect of Henna incorporation on the gel fraction, swelling degree, the morphological features using scanning electron microscopy, fluid drainage ability using pseudo-extracellular body fluids and the bioadhesion characteristics. It was found that, at the time the increment of Henna content slightly decreased the gel fraction, it improves the swelling degree. Scanning electron micrograph showed an adequate porous structure which is an essential criterion to be an ideal wound dressing. The bioadhesion evaluation showed an adequate adhesion performance as well as ease of removal. The microbial penetration test against Staphylococcus aureus and Escherichia coli revealed that Henna dressing is not only a good barrier for bacterial penetration but also it prevents its growth. In vitro cytotoxicity and haemolytic potency evaluation of Henna dressing substantiate its biocompatibility.