Isolation and detection of ingested DNA from the immature stages of Calliphora dubia (Diptera: Calliphoridae): A forensically important blowfly

被引：10

作者：

Carvalho F. ^{[1
]}

Dadour I.R. ^{[1
,3
]}

Groth D.M. ^{[2
]}

Harvey M.L. ^{[1
]}

机构：

[1] Centre Tor Forensic Science, University of Western Australia, Perth, WA

[2] School of Biomedical Sciences, Curtin University, Perth, WA

[3] Centre for Forensic Science, University of Western Australia, Nedlands, WA 6009

来源：

Forensic Science, Medicine, and Pathology | 2005年 / 1卷 / 4期

关键词：

Blowfly; Calliphoridae; DNA extraction; Forensic entomology; Larvae;

D O I：

10.1385/FSMP:1:4:261

中图分类号：

学科分类号：

摘要：

The forensic entomologist frequently bases time since death (TSD) estimation on fly larvae. In some cases, the food source on which these larvae have completed their development may be questionable, and requires verification to ensure the accuracy of the TSD estimation. Ingested DNA may be isolated from the alimentary canal of immature insects. Previous studies have confirmed the ability to extract ingested DNA from the alimentary tract of third instar blowfly larvae. This study considers the potential to detect ingested DNA from immature stages of the blue-bodied blowfly Calliphora dubia (Macquart) that had fed on sheep liver. Individuals from early first instar larvae through day 3 pupae were surface decontaminated, followed by DNA isolation and detection by amplifying the sheep satellite I region. Fragments of 197 basepairs (bp) and 87 bp were successfully isolated and detected in all stages of immatures until 2-day-old pupae, with detection at this stage being unsuccessful on 3-day-old pupae. This study presents a suitable protocol for the isolation and detection of ingested DNA from immature stages of C. dubia. Copyright © 2005 Humana Press Inc. All rights of any nature whatsoever are reserved.

引用

页码：261 / 265

页数：4

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