Development of EST-SSR Markers and Analysis of Genetic Relationship it’s Resources in Hexaploid Oats

被引：4
作者：
Isabel L.P. [1 ]
Park J.-R. [2 ]
Lee G.S. [3 ]
Park G.-H. [4 ]
Kim K.-M. [2 ]
机构：
[1] Ministry of Agriculture and Food Security, National Directoracte of Agricultural Extension (DNEA), Agronomy Engineering, Eduardo Mondlane University, Maputo
[2] Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu
[3] Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, Jeonju
[4] Major in Plant Resources Environment, College of Ecology and Environmental Science, Kyungpook National University, Sangju
来源：
Journal of Crop Science and Biotechnology | 2019年 / 22卷 / 3期
关键词：
Cereal crop; EST (Expressed Sequence Tags); Oat; Seed dispute; SSR (Simple Sequence Repeats);
D O I：
10.1007/s12892-019-0158-0
中图分类号：
学科分类号：
摘要：
Oats are the fifth largest planted area in the world, and are the only cereal crops in the world to be among the top ten in the world. However, since oats are weak in cold weather, only four varieties are cultivated in Korea at present. Therefore, we constructed the DNA profile database using the EST-SSR marker for the oat 73 varieties kept in Korea. When the oat 73 cultivars marketed in Korea were tested with the eight EST-SSR marker developed in this study, 71.62 average alleles were detected per marker and the mean of PIC value was 0.337. According to the polymorphism of microsatellite markers, 73 varieties of oats were classified into 5 ~ 9 groups and most of the genetic resources investigated were identified when EST-SSR marker was used. In the future, EST-SSR markers developed in this study may be used to identify the seed disputes and breeds of oats. In addition, it predicts that it will be usefully used as a means of evaluating the characteristics of oat genetic resources, testing purity and studying the cultivation of varieties. © 2019, Korean Society of Crop Science and Springer.
引用
收藏
页码：243 / 251
页数：8
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