Comparison of methods for sample preparation of individual rat cerebrospinal fluid samples prior to two-dimensional polyacrylamide gel electrophoresis

被引：0
作者：
Sonia Garcia-Rodriguez
Sandro Argüelles Castilla
Alberto Machado
Antonio Ayala
机构：
[1] Universidad de Sevilla,Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia
[2] C/Profesor García González s/n,undefined
来源：
Biotechnology Letters | 2003年 / 25卷
关键词：
rat cerebrospinal fluid; sample preparation; two-dimensional mapping;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Different pre-treatment methods have been compared for two-dimensional mapping of individual rat cerebrospinal fluid samples based on acetone, trichloroacetic acid/acetone and methanol/acetone precipitation of proteins. Acetone precipitation following incubation with DTT gave the highest protein recovery (72%) and the largest number of protein spots (92 ± 4) as well as minimizing the time taken.
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页码：1899 / 1903
页数：4
相关论文
共 53 条
[1]  
Castagna A(2000)Comparative two-dimensional mapping of prion protein isoforms in human cerebrospinal fluid and central nervous system Electrophoresis 23 339-346
[2]  
Campostrini N(2000)Technique for collecting cerebrospinal fluid in the cisterna magna of non-anesthetized rats Brain Res. Protoc. 5 109-114
[3]  
Farinazzo A(2002)Identification of proteins in human cerebrospinal fluid using liquidphase isoelectric focusing as a prefractionation step followed by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry Rapid Commun. Mass Spectrom. 16 2083-2088
[4]  
Zanusso G(2001)Proteome studies of human cerebrospinal fluid and brain tissue using a preparative two-dimensional electrophoresis approach prior to mass spectrometry Proteomics 1 444-452
[5]  
Monaco S(1996)Solubilization of proteins for electrophoretic analyses Electrophoresis 17 813-829
[6]  
Righetti PG(2000)Identification of two-dimensionally separated human cerebrospinal fluid proteins by Electrophoresis 21 2266-2283
[7]  
Consiglio AR(1982)-terminal sequencing, matrix-assisted laser desorption/ionization-mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry J. Biochem. Biophys. Meth. 6 219-227
[8]  
Lucion AB(2000)Effect of 2-mercaptoethanol on pH gradients in isoelectric focusing Electrophoresis 21 2721-2728
[9]  
Davidsson P(2002)Identification of proteins from human cerebrospinal fluid, separated by two-dimensional polyacrylamide gel electrophoresis J. Chromatogr. B 771 167-196
[10]  
Folkesson S(2001)Towards a high resolution separation of human cerebrospinal fluid Electrophoresis 22 3043-3052
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