Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

被引：3

作者：

Jun Q. ^{[1
,4
]}

Xian-zhu X. ^{[1
]}

Song-tao Y. ^{[1
]}

De-sheng L. ^{[2
]}

Gui-xue H. ^{[3
]}

Yu-wei G. ^{[1
]}

He-ting S. ^{[1
]}

Zhong-pen Z. ^{[1
]}

Zhi-jing X. ^{[1
]}

Fang Y. ^{[1
]}

Wen-qi H. ^{[1
]}

Gen H. ^{[1
]}

机构：

[1] Institute of Veterinary, Academy of Military Medical Sciences, Changchun

[2] Research & Conservation Center for Giant Panda of China, Sichuan, Wolong

[3] Department of Animal Science and Technology, Jinling Agricultural University, Changchun

[4] Department of Animal Science and Technology, Tarim University, Xinjiang

来源：

Journal of Forestry Research | 2004年 / 15卷 / 4期

基金：

中国国家自然科学基金;

关键词：

Canine coronavirus; Giant panda; Neutralization test; Serological survey;

D O I：

10.1007/BF02844956

中图分类号：

学科分类号：

摘要：

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda’s sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection. © 2004, Northeast Forestry University.

引用

页码：295 / 297

页数：2

共 17 条

[1]

Appel M., Et al., Canine coronavirus [C], Virus infections of carnivores I, pp. 115-122, (1987)

[2]

Bandai C., Ishiguro S., Masuya N., Et al., Canine coronavirus infections in Japan: virological and epidemiological aspects, J. Vet. Med. Sci., 61, 7, pp. 731-736, (1999)

[3]

Binn L.N., Lazar E.C., Keenan K.P., Et al., Recovery and characterization of a coronavirus from military dogs with diarrhea [J], Proceedings of the Annual Meeting of the US Animal Health Assoc., 78, pp. 359-366, (1974)

[4]

Evermann J.F., Foreyt W., Maag-Miller L., Et al., Acute hemorrhagic enteritis associated with canine coronavirus and parvovirus infection in a captive coyote population [J], J. Am. Vet. Med. Assoc., 177, 9, pp. 784-786, (1980)

[5]

Fensan G., Guixue H., Xianzhu X., Et al., Diagnosis of complex infection of canine distemper virus and canine coronavirus to giant panda by multi-PCR [J], Chinese Journal of Jilin Agricultural University, 25, I, pp. 91-93, (2003)

[6]

Aihua H., Shengfang D., Guihua L., Et al., Mini report on detection of coronavirus in feces of giant panda [J], Chinese Journal of zoonosis, 4, 3, (1996)

[7]

Keenan K.P., Jervis H.R., Marchwicki R.H., Et al., Intestinal infection of neonatal dogs with canine coronavirus 1–71: studies by virologic, histologie, histochemical, and immunofluorescent techniques [J], Am. J. Vet. Res., 37, 3, pp. 247-256, (1976)

[8]

Kelly D.F., Carter S.D., Gaskell C.J., Canine coronavirus infection in the dog following oronasal inoculation [J], Res. Vet. Sci., 51, 1, pp. 11-18, (1991)

[9]

Mainka S.A., Qiu X., He T., Et al., Serologie survey of giant panda (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve, China [J], Journal of Wildlife Disease, 30, 1, pp. 86-89, (1994)

[10]

Pratelli A., Buonavoglia D., Martella V., Et al., Diagnosis of canine coronavirus infection using nested-PCR [J], J.Virol. Meth., 84, 1, pp. 91-94, (2000)

← 1 2 →