A PCR-based assay for differentiating A- and A*-type strains of Xanthomonas citri subsp. citri, the causal agent of Asiatic citrus canker

被引：0
作者：
Fatemeh Yousefi Koupaei
S. Mohsen Taghavi
Hiroshi Shiotani
Shinji Tsuyumu
机构：
[1] Shiraz University,Department of Plant Protection, College of Agriculture
[2] NARO Institute of Fruit Tree Science,Citrus Research Division, Kuchinotsu
[3] Shizuoka University,Faculty of Agriculture
来源：
Journal of General Plant Pathology | 2014年 / 80卷
关键词：
subsp. ; Detection; PCR; A* strain; Asiatic citrus canker;
D O I：
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摘要：
By applying A- and A*-type strains of Xanthomonas citri subsp. citri (Xcc) in a repetitive sequence-based polymerase chain reaction (rep-PCR), two DNA amplicons, one unique to each strain, were evaluated as a probe against the DNA of Xcc strains. Two pairs of primers derived from these amplicons were tested in a PCR analysis. The results confirmed that primers Ms+/Ms− are useful for differentiating A-type from A*-type strains of Xcc. Also, a multiplex PCR with both set of primers can be used to distinguish three groups in Xcc populations: A-type strains and two subgroups of A* strains including Iranian and Thai A* strains.
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页码：85 / 89
页数：4
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