Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation

被引：0
作者：
Wu Meng
Feng Ding
Rui-Ming Wang
Teng-Fei Wang
机构：
[1] State Key Laboratory of Biobased Material and Green Papermaking (LBMP),
[2] Qilu University of Technology (Shandong Academy of Sciences),undefined
[3] Key Laboratory of Shandong Microbial Engineering,undefined
[4] Qilu University of Technology (Shandong Academy of Sciences),undefined
[5] Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education,undefined
[6] Shandong Province,undefined
[7] Qilu University of Technology,undefined
来源：
Scientific Reports | / 10卷
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摘要：
Bacillus licheniformis BL1 was used as a starting strain to construct the recombinant tetramethylpyrazine (TMP)-producing strains by over-expression of the α-acetolactate decarboxylase gene (aldC) and α-acetolactate synthase gene (alsS), named BLC, BLS and BLCS, respectively. Then the addition of acetaldehyde was use to enhance the TMP yield in the fermentation process. During microaerobic fermentation, the aldC-overexpressed BLC strain produced 43.75 g TMP/L which was 15.47% higher than the TMP in culture yielded using the initial BL1 strain. Furthermore, the acetoin yield as TMP precursor similarly rose by 23.06% in BLC recombinant strain. In contrast, the 2,3-BD increased by 23.2% in the recombinant BLCS. TMP produced by BL1 could be bolstered via the supplementation of the acetaldehyde in fermentation medium. This method also has the same effect on the BLC strain.
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