Evaluation of the number of ionogenic groups of inulinase by acid-base titration

被引：0
|
作者：
T. A. Kovaleva
M. G. Holyavka
S. G. Rezvan
S. V. Kozhedub
机构：
[1] Voronezh State University,
来源：
Bulletin of Experimental Biology and Medicine | 2008年 / 145卷
关键词：
inulinase; acid base titration;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Acid base titration showed that Aspergillus awamori inulinase includes 178 asparaginic and glutamic acid residues, 20 histidine, 10 serine, and 34 lysine and tyrosine residues. Denaturation temperature for this enzyme was calculated using analysis of the proportion of stabilizing and destabilizing amino acids in the molecule.
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页码：769 / 771
页数：2
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