Insight into Fluorescence Imaging and Bioorthogonal Reactions in Biological Analysis

被引：0
作者：
Yuexiang Ma
Qinhua Chen
Xiaoyan Pan
Jie Zhang
机构：
[1] Xi’an Jiaotong University,School of Pharmacy, Health Science Center
[2] Shenzhen Baoan Authentic TCM Therapy Hospital,Department of Pharmacy
来源：
Topics in Current Chemistry | 2021年 / 379卷
关键词：
Fluorescence probe; Bioorthogonal reaction; Action mechanism; Biological analysis;
D O I：
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中图分类号：
学科分类号：
摘要：
Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.
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