Cytotoxicity of methylsulfonylmethane on gastrointestinal (AGS, HepG2, and KEYSE-30) cancer cell lines

被引：18
作者：
Jafari N. [1 ]
Bohlooli S. [1 ]
Mohammadi S. [1 ]
Mazani M. [2 ]
机构：
[1] Department of Pharmacology, School of Medicine, Ardabil University of Medical Science, Ardabil 56197, University Street
[2] Department of Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil
来源：
Journal of Gastrointestinal Cancer | 2012年 / 43卷 / 3期
关键词：
Apoptosis; Cancer cell lines; Cytotoxicity; Methylsulfonylmethane; MSM;
D O I：
10.1007/s12029-011-9291-z
中图分类号：
学科分类号：
摘要：
Purpose: This study was conducted to assay cytotoxic effects of methylsulfonylmethane (MSM) on gastrointestinal cancer cell lines. Methods: Human gastric carcinoma (AGS), human hepatocellular carcinoma (HepG2), and human esophageal squamous cell carcinoma (KYSE-30) cancer cell lines were treated by MSM and incubated for 24, 48, and 72 h. Cytotoxicity was examined through MTT, neutral red uptake, and protein measurement assays. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic cell detection. A diamidino-2-phenylindole staining method was used to analysis cell cycle by flow cytometry. Results: IC50 of MSM on AGS, HepG2, and KYSE-30 cell lines were 28.04, 21.87 and 27.98 mg/ml after 72 h, respectively. The EB/AO staining showed an increase in apoptotic cells. Cell cycle analysis showed a significant increase in cell density at G2/M phase. Conclusion: MSMhad cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible. This study suggests that MSM may induce cytotoxic effect on gastrointestinal cancer cell lines by apoptosis and cell cycle arrest. © Springer Science+Business Media, LLC 2011.
引用
收藏
页码：420 / 425
页数：5
相关论文
共 23 条
  • [1] Monograph, Altern. Med. Rev., 8, 4, pp. 438-441, (2003)
  • [2] Christy M., MSM -The Super-supplement of the Decade, (1997)
  • [3] Jacob S., Appleton J., MSM the Definitive Guide: The Nutritional Breakthrough for Arthritis, Allergies and More, (2002)
  • [4] Beilke M.A., Collins-Lech C., Sohnle P.G., Effects of dimethyl sulfoxide on the oxidative function of human neutrophils, J Lab Clin Med., 110, 1, pp. 91-96, (1987)
  • [5] McCabe D., Et al., Polar solvents in the chemoprevention of dimethylbenzanthracene-induced rat mammary cancer, Arch Surg., 121, 12, pp. 1455-1459, (1986)
  • [6] Caron J.M., Et al., Methyl sulfone induces loss of metastatic properties and reemergence of normal phenotypes in a metastatic cloudman S-91 (M3) murine melanoma cell line, PLoS One., 5, 8, (2010)
  • [7] Cox W.I., Specter S., Friedman H., Susceptibility of Friend erythroleukemia cells to natural cytotoxicity after in vitro treatment with dimethyl sulfoxide, Proc Soc Exp Biol Med., 169, 3, pp. 337-342, (1982)
  • [8] Ebisuzaki K., Aspirin and methylsulfonylmethane (MSM): A search for common mechanisms, with implications for cancer prevention, Anticancer Res., 23, 1 A, pp. 453-458, (2003)
  • [9] Satia J.A., Et al., Associations of herbal and specialty supplements with lung and colorectal cancer risk in the VITamins and Lifestyle Study, Cancer Epidemiol Biomarkers Prev., 18, 5, pp. 1419-1428, (2009)
  • [10] Alam S.S., Layman D.L., Dimethyl sulfoxide as a cholesterollowering agent in cultured fibroblasts exposed to low density lipoproteins, Biochim Biophys Acta., 710, 3, pp. 306-313, (1982)
123