A PCR-based method to detect Sclerotium hydrophilum in infected rice leaf sheaths

被引：0

作者：

C. J. Hu

Y. R. Li

Y. W. Wei

S. L. Huang

机构：

[1] Guangxi Academy of Agricultural Sciences,Plant Protection Research Institute

[2] Guangxi Crop Genetic Improvement and Biotechnology Lab,undefined

[3] Nanyang Normal University,undefined

[4] Guangxi University,undefined

来源：

Australasian Plant Pathology | 2008年 / 37卷

关键词：

Internal Transcribe Spacer; Australasian Plant Pathology; Sheath Blight; Rice Sheath Blight; Crop Genetic Improvement;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

An oligonucleotide primer pair set based on internal transcribed spacer regions of rDNA for PCR, which gives the amplicon for only the DNA from Sclerotium hydrophilum was designed. Tests on the specificity of the primers concerned showed that a single product of 540 bp was amplified from the DNA of S. hydrophilum but not from the DNA of any other species responsible for sheath disease in rice, suggesting that the primer pair could be used to develop a PCR-based diagnostic method to provide a rapid and accurate molecular detection of this pathogen in rice leaf sheath.

引用

页码：40 / 42

页数：2

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