Agrobacterium-mediated transformation of centipedegrass (Eremochloa ophiuroides [Munro] Hack.)

被引：0
作者：
Mingxi Liu
Shaoyun Lu
Lin Liu
Jiali Tan
Zhenfei Guo
机构：
[1] South China Agricultural University,State Key Laboratory for Conservation and Utilization of Subtropical Agro
[2] South China Agricultural University,Bioresources
来源：
Plant Cell, Tissue and Organ Culture (PCTOC) | 2012年 / 109卷
关键词：
-mediated transformation; Centipedegrass; Turfgrass;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Centipedegrass (Eremochloa ophiuroides [Munro] Hack.) is an important warm-season turfgrass and pasture grass. Due to availability of an efficient regeneration system, this study was undertaken to develop an Agrobacterium-mediated transformation system for centipedegrass for potential use in its genetic improvements. Embryogenic calli were co-cultivated with Agrobacterium strain EHA105 harboring pCAMBIA1301, followed by selection of hygromycin B (hyg B)-resistant callus and regenerated plantlets. The transformants were analyzed using PCR, DNA blot, RNA blot and semi-quantitative RT-PCR. Two transformant lines, lines 2 and 6, showed integration and expression of the hyg B resistance gene hpt. Line 2 revealed a single integration of the hpt transgene, while line 6 revealed two integration sites of hpt. Both lines exhibited resistance to hyg B, although line 2 showed higher level of expression of hpt.
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页码：557 / 563
页数：6
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