Channel Catfish Follicle-Stimulating Hormone and Luteinizing Hormone: Complementary DNA Cloning and Expression During Ovulation

被引：0

作者：

Zhanjiang Liu

Soonhag Kim

Attila Karsi

机构：

[1] Fish Molecular Genetics and Biotechnology Laboratory,

[2] Department of Fisheries and Allied Aquacultures,undefined

[3] and Program of Cell and Molecular Biosciences,undefined

[4] Auburn University,undefined

[5] Auburn,undefined

[6] AL 36849,undefined

[7] U.S.A.,undefined

来源：

Marine Biotechnology | 2001年 / 3卷

关键词：

Key words: spawning, induction, gene, expression, pituitary, reproduction.;

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摘要：

Gonadotropins are important regulators of reproduction. To develop molecular resources for production of recombinant gonadotropins, we have cloned and sequenced complementary DNA encoding the channel catfish (Ictalurus punctatus) follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which encode 132 and 140 amino acid proteins, respectively. The deduced amino acid sequences of the catfish FSH and LH are highly conserved with those cloned from other teleosts. Both the FSH and the LH were highly induced during ovulation after injection of carp pituitary extract. Taken together with our previous findings of enhanced expression of growth hormone and other pituitary hormones, this research suggests that a hormonal cocktail may be needed for more efficient manipulation of catfish reproduction. The availability of the catfish FSH and LH cDNAs provides the opportunity for production of immunologically or biologically active recombinant gonadotropins for the study of catfish reproductive physiology and the manipulation of artificial spawning for aquaculture.

引用

页码：590 / 599

页数：9