Expression of foreign proteins in a Vibrio cholerae vaccine strain using the stationary phase hemagglutinin/protease promoter

被引：0
|
作者：
Anupam Hazra
Anisia J. Silva
Jorge A. Benitez
机构：
[1] Morehouse School of Medicine,Department of Microbiology, Biochemistry and Immunology
来源：
Biotechnology Letters | 2007年 / 29卷
关键词：
Hemagglutinin/protease; Protein expression; Vaccine development;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The use of the hemagglutinin(HA)/protease promoter and secretion signals to drive expression and secretion of a foreign antigen in a live genetically attenuated cholera vaccine candidate is demonstrated. A Vibrio cholerae vaccine strain, containing a HA/protease-tetanus toxin C fragment (TCF) fusion, produced soluble-and cell-associated TCF. The fraction of TCF secreted to the culture medium was degraded unless expressed in a HA/protease-defective vaccine strain. Comparison of the hapA promoter with the strong Tac promoter using quantitative real time PCR revealed that at least five times more TCF mRNA was produced when expressed from the hapA promoter.
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页码：1093 / 1097
页数：4
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