Champacaine A, 7-methoxy-1,2,3,4-tetrahydroisoquinolin-1-one, thalifoline, N-methylcorydaldine, liriodenine, 11,13-dehydrolanuginolide, (+)-syringaresinol, (+)-epi-syringaresinol, (+)-dia-syringaresinol, (+)-sesamin, N-trans-feruloyltyramine, syringic acid, syringaldehyde, 2,6-dimethoxy-p-benzoquinone, β-sitostenone, and stigmasta-4,22-dien-3-one were isolated from the roots of Michelia champaca L. (Magnoliaceae). Among them, champacaine A is a new aristolactam alkaloid. The structures of these compounds were characterized and identified by spectral analyses.